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UTMB Proteomics Center

The UTMB NHLBI Center for Airway Inflammation consists of a multi-disciplinary team of scientists and physician-scientists to study protein expression associated with signaling pathways important in airway inflammation. In addition, the Center is engaged in the development of innovative technologies for application to proteomics research. It is the result of an NHLBI initiative to establish centers to enhance and develop innovative proteomics technologies and apply them to biological questions relevant to heart, lung, blood, and sleep health and disease.

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Proteomics Bioinformatics Core

As part of the Proteomics Section of the Biomolecular Resource Facility and consistent with its mission, the goal of the Proteomics Bioinformatics Core is to assist researchers at UTMB in their analyses of gels and other proteomics separation technologies.

  • The Protein Bioinformatics Core provides informatics tools, including statistical tools, for the analysis of 1D and 2D electrophoresis gels utilizing a software suite from Totallab (formerly Nonlinear Dynamics) and sample tracking, data management and access tools, using software from GenoLogics (Proteus LIMS). The BRF is a major Nonlinear and GenoLogics partner and collaborates with these manufacturers in the development of their software.
  • The Bioinformatics Core interacts extensively with other BRF Cores, and UTMB Centers and Departments and has integrated the Progenesis software into high-end user groups. For example, the BRF has installed the Editor Progenesis software at a number of sites across the UTMB campus.
  • Group training sessions are scheduled as needed, typically every six months.

 

Mass Spectrometry Core

The aim of the UTMB Mass Spectrometry Core (MSC) is to assemble state-of-the-art instrumentation and highly skilled technical staff to provide experimental capabilities required to support a broad range of molecular-level research. Mass spectrometry (MS) plays an increasingly important role in molecular-level research, and it is central to ‘omics’ research.  The MSC is well-equiped to facilitate studies with a suite of LC-MS systems including the ThermoScientific Fusion Orbitrap MS, the Sciex 5600 TripleTOF, 6500 Qtrap, and 5800 MALDI TOF/TOF.

  • Molecular weight determination of biomolecules such as lipids, metabolites, peptides and proteins.
  • Protein identification from gel plugs or bands and solutions using enzymatic digestion followed by MS or MS/MS using either MALDI or LCMS.
  • Proteomics: identification of large numbers of proteins from complex mixtures using LCMS. Can include quantitative measurements.
  • Identification of post-translational modifications.
  • Differential quantitation by label-free (DIA, DDA, or SWATH), iTRAQ, TMT, or SILAC using the Thermo Orbitrap Fusion or Sciex 5600+ TripleTof.
  • Quantitation of molecules using Selected Reaction Monitoring (SRM) or multi-reaction monitoring (MRM) on a 6500 QTrap.
  • Advanced informatics from proteomics datasets: Beyond Mascot and Sequest output.